雄烯二醇(AED)酶联免疫吸附检测试剂盒

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Product Name:雄烯二醇(AED)酶联免疫吸附检测试剂盒
Alternative Names:5-AED; 5AED; 5-Androstenediol; Androst-5-Ene-3Beta,17Beta-Diol
Species:General
Assay Type:Competitive Inhibition
Sensitivity:55.5 pg/mL
standard:10000 pg/mL
Detection range:156.25-10000 pg/mL
Sample type:serum, plasma and other biological fluids
Assay length:2h
Research Area:Endocrinology;Reproductive science;Hormone metabolism;
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AED protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AED. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AED in the samples is then determined by comparing the OD of the samples to the standard curve.

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