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> 小鼠周期素依赖性激酶5(CDK5)酶联免疫吸附检测试剂盒
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小鼠周期素依赖性激酶5(CDK5)酶联免疫吸附检测试剂盒  

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Product Name：小鼠周期素依赖性激酶5(CDK5)酶联免疫吸附检测试剂盒
Alternative Names：PSSALRE; CDKN5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.053 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：tissue homogenates, cell lysates and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;Enzyme & Kinase;Neuro science;Developmental science;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CDK5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CDK5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CDK5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CDK5 in the samples is then determined by comparing the OD of the samples to the standard curve.

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