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> 小鼠蛋白磷酸酶1调节因子亚基15A(PPP1R15A)酶联免疫吸附检测试剂盒
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小鼠蛋白磷酸酶1调节因子亚基15A(PPP1R15A)酶联免疫吸附检测试剂盒  

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Product Name：小鼠蛋白磷酸酶1调节因子亚基15A(PPP1R15A)酶联免疫吸附检测试剂盒
Alternative Names：PPP1-R15A; PPP1R15-A; PPP1-R15-A; GADD34; Growth Arrest And DNA-Damage-Inducible 34; Myeloid differentiation primary response protein MyD116 homolog
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.057 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：tissue homogenates, cell lysates and other biological fluids
Assay length：3.5h
Research Area：Developmental science;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PPP1R15A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPP1R15A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PPP1R15A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PPP1R15A in the samples is then determined by comparing the OD of the samples to the standard curve.

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