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> 小鼠氯化物细胞内通道蛋白1(CLIC1)酶联免疫吸附检测试剂盒
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小鼠氯化物细胞内通道蛋白1(CLIC1)酶联免疫吸附检测试剂盒  

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Product Name：小鼠氯化物细胞内通道蛋白1(CLIC1)酶联免疫吸附检测试剂盒
Alternative Names：G6; NCC27; p64CLCP; hRNCC; Chloride channel ABP; Nuclear chloride ion channel 27; Regulatory nuclear chloride ion channel protein
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.057 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids.
Assay length：3.5h
Research Area：Signal transduction;Metabolic pathway;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CLIC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CLIC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CLIC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CLIC1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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