大鼠UDP葡萄糖醛酸转移酶2家族多肽B7(UGT2B7)酶联免疫吸附检测试剂盒  

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Product Name:大鼠UDP葡萄糖醛酸转移酶2家族多肽B7(UGT2B7)酶联免疫吸附检测试剂盒
Alternative Names:UGTB2B9; UDPGTh-2; 3,4-catechol estrogen-specific UDPGT
Species:Rat
Assay Type:Sandwich
Sensitivity:0.057 ng/mL
standard:10 ng/mL
Detection range:0.16-10 ng/mL
Sample type:Tissue homogenates and other biological fluids.
Assay length:3.5h
Research Area:Enzyme & Kinase;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat UGT2B7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat UGT2B7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat UGT2B7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat UGT2B7 in the samples is then determined by comparing the OD of the samples to the standard curve.