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> 小鼠N-型电压依赖钙离子通道α1B亚基(CACNa1B)酶联免疫吸附检测试剂盒
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小鼠N-型电压依赖钙离子通道α1B亚基(CACNa1B)酶联免疫吸附检测试剂盒  

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Product Name：小鼠N-型电压依赖钙离子通道α1B亚基(CACNa1B)酶联免疫吸附检测试剂盒
Alternative Names：CACNN; CACNL1A5; BIII; Brain calcium channel III; Calcium channel, L type, alpha-1 polypeptide isoform 5; Voltage-gated calcium channel subunit alpha Cav2.2
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.055 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids.
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CACNa1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CACNa1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CACNa1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CACNa1B in the samples is then determined by comparing the OD of the samples to the standard curve.

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