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> 大鼠成纤维细胞激活蛋白α(FAPa)酶联免疫吸附检测试剂盒
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大鼠成纤维细胞激活蛋白α(FAPa)酶联免疫吸附检测试剂盒  

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Product Name：大鼠成纤维细胞激活蛋白α(FAPa)酶联免疫吸附检测试剂盒
Alternative Names：APCE; SIMP; DPPIV; Seprase; 170 kDa melanoma membrane-bound gelatinase; Post-proline cleaving enzyme; Serine integral membrane protease; Surface-expressed protease
Species：Rat
Assay Type：Sandwich
Sensitivity：0.123 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：serum, plasma, tissue homogenates and other biological fluids
Assay length：3.5h
Research Area：Tumor immunity;Dermatology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FAPa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FAPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FAPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FAPa in the samples is then determined by comparing the OD of the samples to the standard curve.

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