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> 山羊白介素12 p40(IL-12p40)酶联免疫吸附检测试剂盒
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山羊白介素12 p40(IL-12p40)酶联免疫吸附检测试剂盒  

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Product Name：山羊白介素12 p40(IL-12p40)酶联免疫吸附检测试剂盒
Alternative Names：IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit
Species：Goat
Assay Type：Sandwich
Sensitivity：6.3 pg/mL
standard：1000 pg/mL
Detection range：15.63-1000 pg/mL
Sample type：Serum, plasma and other biological fluids.
Assay length：3.5h
Research Area：Cytokine;Infection immunity;Immune molecule;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

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