猪生长抑素(SST)酶联免疫吸附检测试剂盒
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Alternative Names:GHIH; SRIF; SMST; GHRIH; GHRIF; Growth Hormone Releasing Inhibiting Hormone; Somatotropin Release-Inhibiting Factor; Somatostatin-14; Somatostatin-28
Species:Pig
Assay Type:Competitive Inhibition
Sensitivity:2.79 pg/mL
standard:500 pg/mL
Detection range:7.82-500 pg/mL
Sample type:Serum, plasma and other biological fluids.
Assay length:2h
Research Area:Tumor immunity;Endocrinology;Hormone metabolism;
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig SST protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig SST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig SST in the samples is then determined by comparing the OD of the samples to the standard curve.
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