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> 人肽基脯氨酰顺反式异构酶NIMA相互作用蛋白1(PIN1)酶联免疫吸附检测试剂盒
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人肽基脯氨酰顺反式异构酶NIMA相互作用蛋白1(PIN1)酶联免疫吸附检测试剂盒  

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Product Name：人肽基脯氨酰顺反式异构酶NIMA相互作用蛋白1(PIN1)酶联免疫吸附检测试剂盒
Alternative Names：DOD; Protein(Peptidyl-Prolyl Cis/Trans Isomerase)NIMA-Interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; Rotamase Pin1
Species：Human
Assay Type：Sandwich
Sensitivity：0.055 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：tissue homogenates, cell lysates and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIN1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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