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> 人味觉受体2型成员38(TAS2R38)酶联免疫吸附检测试剂盒
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人味觉受体2型成员38(TAS2R38)酶联免疫吸附检测试剂盒  

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Product Name：人味觉受体2型成员38(TAS2R38)酶联免疫吸附检测试剂盒
Alternative Names：PTC; T2R61; Phenylthiocarbamide Tasting; PTC bitter taste receptor
Species：Human
Assay Type：Sandwich
Sensitivity：0.056 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay length：3.5h
Research Area：CD & Adhesion molecule;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TAS2R38. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TAS2R38. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TAS2R38, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TAS2R38 in the samples is then determined by comparing the OD of the samples to the standard curve.

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