小鼠食欲素(OX)酶联免疫吸附检测试剂盒  

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Product Name:小鼠食欲素(OX)酶联免疫吸附检测试剂盒
Alternative Names:HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor
Species:Mouse
Assay Type:Competitive Inhibition
Sensitivity:2.74 pg/mL
standard:500 pg/mL
Detection range:7.82-500 pg/mL
Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:2h
Research Area:Neuro science;
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse OX protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OX in the samples is then determined by comparing the OD of the samples to the standard curve.