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A-802715  ≥98%

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包装规格：1mg 5mg 10mg 20mg in glass bottle
溶解性：溶于DMSO（190mg/mL 超声)
产品描述：基本信息 产品编号： A11745 产品名称： A-802715 CAS: 107767-58-8   储存条件 粉末 -20℃ 四年     分子式： C16H26N4O3 溶于液体 -80℃ 六个月 分子量 322.40 -20℃ 一个月 化学名：  1-(5-hydroxy-5-methylhexyl)-3-methyl-7-propylpurine-2,6-dione Solubility (25°C)：   体外:   DMSO 190mg/mL (589.33mM; Need ultrasonic) Ethanol   Water   体内(现配现用): 1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 7.5mg/mL (23.26mM); Clear solution 此⽅案可获得 ≥ 7.5mg/mL (23.26mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 75.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 2.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 7.5mg/mL (23.26mM); Clear solution 此⽅案可获得 ≥ 7.5mg/mL (23.26mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 75.0mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。 3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 7.5mg/mL (23.26mM); Clear solution 此⽅案可获得 ≥ 7.5mg/mL (23.26mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取 100μL 75.0mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。 ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 3.1017mL 15.5087mL 31.0174mL 5mM 0.6203mL 3.1017mL 6.2035mL 10mM 0.3102mL 1.5509mL 3.1017mL   生物活性 产品描述 一种甲基黄嘌呤衍生物，TD50为 0.9-1.1mM。 体外研究 The toxicity of the methylxanthine derivative A802715 is determined against the two human melanoma lines, Be11 and MeWo, and against the two human squamous cell carcinoma lines, 4197 and 4451, by vital dye staining assay. A802715 has a TD50 of 0.9-1.1mM and is the most toxic. In p53 wt cells BrdU incorporations show that the irradiation-induced suppression of S-phase entry is strongly enhanced by A802715. A802715 prolongs the G2/M block or remain ineffective depending on the p53 status of the cell line   推荐实验方法(仅供参考) Cell Assay DNA analysis is performed using a FACScan flow cytometer emitting a 488 nm beam. Red fluorescence from PI emission is collected as a linear signal through a 600 nm bandpass filter and recorded as a measure of total DNA content. Processing the red fluorescence into height, area and width (doublet discrimination mode) eliminated cell doublets. Data are collected in list mode and 10000 events are recorded per sample and displayed as a frequency distribution histogram. Estimates of the percentages of cells in the different periods of postirradiation incubation with marker statistics reveal the time at which the G2/M block is maximally expressed. These times are used for each cell line as a starting point at which the methylxanthine drugs were added. Cell debris, nuclei doublets and triplets were excluded by gating
保存条件：-20℃

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