生物试剂
>
生化试剂
>
信号通路
> MP7
加入收藏 取消收藏

MP7  ≥98%

促销价¥{{model.attbuying.price}}

市场价¥0.00

累计销量0 累计评价0 人评论

别称 {{model.alias}}
中文名称 {{model.name}}
英文名称 {{model.enname}}
品牌 {{model.brand}}
CAS {{model.cas}}
分子式
分子量 {{model.molecularweight}}
MDL {{model.mdl}}
货号 {{model.procode}}
数量

+ -

件 库存{{model.attbuying.number}}件
立即购买 加入购物车 下载说明书

看了又看
包装规格：5mg 10mg 50mg 100mg in glass bottle
溶解性：溶于DMSO(≥100mg/mL）
产品描述：基本信息 产品编号： M11674 产品名称： MP7 CAS: 1001409-50-2   储存条件 粉末 -20℃ 四年     分子式： C28H22F2N4O4 溶于液体 -80℃ 6个月 分子量： 516.50 -20℃ 1个月 化学名：  1-[(3,4-difluorophenyl)methyl]-2-oxo-N-[(1R)-2-[(2-oxo-1,3-dihydrobenzimidazol-5-yl)oxy]-1-phenylethyl]pyridine-3-carboxamide；PDK1 inhibitor Solubility (25°C)：   体外:   DMSO   Ethanol   Water   体内(现配现用):   ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 1.9361mL 9.6805mL 19.3611mL 5mM 0.3872mL 1.9361mL 3.8722mL 10mM 0.1936mL 0.9681mL 1.9361mL   生物活性 产品描述 一种磷酸肌醇依赖性激酶-1 (PDK1)抑制剂。 靶点 PDK1 体外研究 Cell counting of U87MG-derived glioma stem cells (GSCs) confirms that Alisertib and, to a minor extent, MP7 (PDK1 inhibitor) are able to decrease the number of viable cells. When combined together, GSC viability is further reduced with respect to single-treated cells. As observed in U87MG cells, when used at the highest concentrations (i.e.,1.5μM Alisertib and 2.5μM MP7),a significant enhancement in the number of dead cells is evidenced.Following 72h treatment,MP7 alone does not show a significant inhibition of glioblastoma multiforme (GBM) proliferation.MP7 has been shown to have only minimal effects on monolayer cell growth in several cancer cell lines,with IC50 values in the micromolar range.   推荐实验方法(仅供参考) 细胞实验:   The human GBM cells (i.e.,U87MG,U343MG,or ANGM-CSS) or the respective GSCs are seeded and incubated for the indicated times with the indicated concentrations of SA16 (1nM to 100μM),MP7 (2.5nM,25nM,250nM and 2.5μM),or Alisertib.When indicated,cells are treated with MP7 and Alisertib in combination.To verify GSC chemoresistance,U87MG or GSCs are incubated with 50μM TMZ for 72h.For the long-term treatment of cells,NSC or complete medium containing drugs is replaced every 3 days.Cell proliferation is determined using the MTS assay:the dehydrogenase activity in active mitochondria reduces MTS to the soluble formazan product,whose absorbance at 490nm is measured with an automated plate reader.The mean background from each test condition is subtracted,and the data are expressed as the percentage of untreated cells (control).IC50 values are derived from the sigmoid dose-response curve.The percentage of inhibition is calculated as 100% minus the percentage of cell proliferation.
保存条件：-20℃

猜你喜欢