生物试剂
>
生化试剂
>
信号通路
> Soraprazan
加入收藏 取消收藏

Soraprazan  ≥98%

促销价¥{{model.attbuying.price}}

市场价¥0.00

累计销量0 累计评价0 人评论

别称 {{model.alias}}
中文名称 {{model.name}}
英文名称 {{model.enname}}
品牌 {{model.brand}}
CAS {{model.cas}}
分子式
分子量 {{model.molecularweight}}
MDL {{model.mdl}}
货号 {{model.procode}}
数量

+ -

件 库存{{model.attbuying.number}}件
立即购买 加入购物车 下载说明书

看了又看
包装规格：5mg in glass bottle
溶解性：溶于DMSO(≥150mg/ml)
产品描述：基本信息 产品编号： S10983 产品名称： Soraprazan CAS: 261944-46-1   储存条件 粉末 -20℃ 四年     分子式： C21H25N3O3 溶于液体 -80℃ 6个月 分子量： 367.44 -20℃ 1个月 化学名：  (7r,8r,9r)-7-(2-methoxyethoxy)-2,3-dimethyl-9-phenyl-7,8,9,10-tetrahydroimidazo[1,2-h][1,7]naphthyridin-8-ol Solubility (25°C)：   体外:   DMSO   Ethanol   Water   体内(现配现用):   ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 2.7215mL 13.6077mL 27.2153mL 5mM 0.5443mL 2.7215mL 5.4431mL 10mM 0.2722mL 1.3608mL 2.7215mL   生物活性 产品描述 一种选择性的钾通道竞争性的H,K-ATPase(Ki=6.4nM)的抑制剂 靶点 Proton Pump   体外研究 Soraprazan (BYK61359) is a potent inhibitor of gastric H,K-ATPase,with an IC50 of 0.1μM when measured in ion-leaky vesicles in the presence of 1mM potassium.Soraprazan (BYK61359) also effectively inhibits dibutyryl cAMP-stimulated [14C]AP accumulation in isolated gastric glands with an IC50 of 0.19μM (0.09-0.40μM geometric mean from n=6 with 95% confidence limits). 体内研究 Soraprazan (1-27μmol/kg;p.o.) shows rapid and consistent inhibition of acid secretion in dog.   推荐实验方法(仅供参考) 激酶实验： [3H]Soraprazan binding studies are carried out at 20℃.In saturation experiments to determine the Kd value,ion-leaky gastric vesicles (0.01-0.02mg/mL) are resuspended in a buffer composed of 20mM Tris-HCl,pH 7.0,2mM MgCl2,and 2mM ATP (pH 7.0 by Tris) in the presence of increasing concentrations of [3H]soraprazan (0.1nM-1μM).Nonspecific binding is determined in the presence of a 100 fold excess of unlabeled soraprazan over the concentration range of [3H]soraprazan used.The enzyme suspension (1mL) is incubated at 20℃ for 30 min and rapidly filtered through a nitrocellulose membrane filter (0.45μM) prewet with a solution composed of 20mM Tris-HCl,pH 7.0,10% polyethylene glycol 3350 that is placed on top of a glass fiber filter.The membrane is ished five times with 2.5mL of a buffer composed of 20mM Tris-HCl,pH 7.0,and 10% polyethylene glycol 3350 to remove unbound inhibitor.The membrane is put into a 20-mL scintillation vial,dimethylacetamide (0.5mL) is added to dissolve the membrane,and 14mL of scintillation solvent is added and counted.Binding of [3H]soraprazan is determined by subtracting the nonspecific binding of [3H]soraprazan,obtained in the presence of the 100-fold excess of nonradioactive soraprazan,from the amounts of [3H]soraprazan bound to the membrane in the absence of the cold inhibitor.
保存条件：-20℃

猜你喜欢