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> 人热稳定性肠毒素受体(GUCY2C)酶联免疫吸附检测试剂盒
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人热稳定性肠毒素受体(GUCY2C)酶联免疫吸附检测试剂盒

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Product Name：人热稳定性肠毒素受体(GUCY2C)酶联免疫吸附检测试剂盒
Alternative Names：DIAR6; GC-C; GUC2C; MECIL; MUCIL; STAR
Species：Human
Assay Type：Sandwich
Sensitivity：0.052 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma, tissue homogenates
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GUCY2C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GUCY2C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GUCY2C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GUCY2C in the samples is then determined by comparing the OD of the samples to the standard curve.

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