大鼠前列腺素F2α(PGF2a)酶联免疫吸附检测试剂盒

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Product Name:大鼠前列腺素F2α(PGF2a)酶联免疫吸附检测试剂盒
Alternative Names:8-isoprostane; 8-iso-PGF2α
Species:Rat
Assay Type:Competitive Inhibition
Sensitivity:3.71 pg/mL
standard:800 pg/mL
Detection range:12.5-800 pg/mL
Sample type:Serum, plasma, saliva and other biological fluids.
Assay length:2h
Research Area:Metabolic pathway;Cardiovascular biology;Hormone metabolism;
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PGF2α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.

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