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> 小鼠卵清蛋白特异性IgG1(OVA sIgG1)酶联免疫吸附检测试剂盒
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小鼠卵清蛋白特异性IgG1(OVA sIgG1)酶联免疫吸附检测试剂盒

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Product Name：小鼠卵清蛋白特异性IgG1(OVA sIgG1)酶联免疫吸附检测试剂盒
Alternative Names：OVA sIgG1; Ovalbumin specific Immunoglobulin G1
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.108 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Cell Biology
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OVA sIgG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OVA sIgG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OVA sIgG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OVA sIgG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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