Product Name：兔固醇调节元件结合转录因子1(SREBP1)酶联免疫吸附检测试剂盒
Alternative Names：SREBF1; BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1
Species：Rabbit
Assay Type：Sandwich
Sensitivity：0.059 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids
Assay length：3.5h
Research Area：Cardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit SREBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit SREBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit SREBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit SREBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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