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> 大鼠过氧蛋白同源物(PXDN)酶联免疫吸附检测试剂盒
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大鼠过氧蛋白同源物(PXDN)酶联免疫吸附检测试剂盒

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Product Name：大鼠过氧蛋白同源物(PXDN)酶联免疫吸附检测试剂盒
Alternative Names：PRG2; PXN; MG50; VPO; VPO1; Melanoma-associated antigen MG50; Vascular peroxidase 1; p53-responsive gene 2 protein
Species：Rat
Assay Type：Sandwich
Sensitivity：0.053 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Enzyme & Kinase;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PXDN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PXDN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PXDN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PXDN in the samples is then determined by comparing the OD of the samples to the standard curve.

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