鸟苷(GS)酶联免疫吸附检测试剂盒

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Product Name:鸟苷(GS)酶联免疫吸附检测试剂盒
Alternative Names:Guanine Riboside
Species:General
Assay Type:Competitive Inhibition
Sensitivity:78.7 pg/mL
standard:20000 pg/mL
Detection range:312.5-20000 pg/mL
Sample type:biological agents
Assay length:2h
Research Area:Signal transduction;Metabolic pathway;
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with GS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GS in the samples is then determined by comparing the OD of the samples to the standard curve.

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