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S-2474  

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产品描述：基本信息 产品编号： S11007 产品名称： S-2474 CAS: 158089-95-3   储存条件 粉末 -20℃ 四年 分子式： C20H31NO3S 溶于液体 -80℃ 二年 分子量： 365.53     化学名：  (E)-5-(3,5-di-tert-butyl-4-hydroxybenzylidene)-2-ethylisothiazolidine 1,1-dioxide Solubility (25°C)：   体外:   DMSO   Ethanol   Water   体内(现配现用):   ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   生物活性 产品描述 COX-2和5-lipoxygenase(5-LO)的抑制剂，在人完整的细胞中，对COX-2和COX-1的IC50值分别为11nM和27μM，为一种非甾体抗炎药。 靶点 COX-2 11nM (IC50) COX-1 27μM (IC50) 5-LO   体外研究 S-2474 is an inhibitor of COX-2 and 5-lipoxygenase,with IC50s of 11nM and 27μM for COX-2 and COX-1.S-2474 prevents neurons from Aβ-induced cell death significantly in a concentration-dependent manner (IC50 =26±12nM).S-2474 (10μM) completely inhibits Aβ(25-35)-induced neuronal cell death.S-2474 also shows neuroprotective effects in the Aβ(1-40)-induced neuronal cell death.S-2474 inhibits the PGD2 generation in a concentration dependent manner (IC50=69.8±21.9nM).S-2474 (10μM) lowers the elevated level of PGD2 significantly and reduces radicals from Aβ(25-35)-treated neurons.S-2474 significantly prevents neurons from undergoing sPLA2-IIA-induced cell death.S-2474 completely ameliorates sPLA2-IIA-induced apoptotic features such as the condensation of chromatin and the fragmentation of DNA.Moreover,S-2474 significantly inhibits the sPLA2-IIA-induced generation of PGD2.S-2474 inhibits sPLA2-IIA-induced neuronal cell death in a concentration-dependent manner (IC50=94nM).   推荐实验方法(仅供参考) 细胞实验:   Experiments are principally performed in the two conditions as follows.(i) Neurons (2.5×105 cells/cm2) are treated with 10μM Aβ(25-35) or Aβ(1-40) in the presence or absence of S-2474 at 37℃.Vehicle controls are treated with culture medium containing 1% deionized water and 0.1% DMSO.Aβ controls are treated with culture medium containing 10μM Aβ(25-35) and 0.1% DMSO.(ii) Neurons (2.5×105 cells/cm2) are treated with eicosanoids at 37℃.Vehicle controls are treated with culture medium containing 0.1% ethanol.Two different methods are employed for assessment of neurotoxicity of Aβ.First,the  3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide dye (MTT) reduction assay reflecting mitochondrial succinate dehydrogenase activity is employed.Second,residual cells are counted according to morphologic criteria;neurons with intact neurites and a smooth,round soma are considered viable,whereas those with degenerated neurites and an irregular soma are considered nonviable.
保存条件：-20℃

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