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SN6  ≥98%

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包装规格：5mg 25mg 100mg in glass bottle
溶解性：溶于DMSO(60mg/ml 超声）
产品描述：基本信息 产品编号： S10966 产品名称： SN6 CAS: 415697-08-4   储存条件 粉末 -20℃ 四年     分子式： C20H22N2O5S 溶于液体 -80℃ 6个月 分子量： 402.46 -20℃ 1个月 化学名：  2-[[4-[(4-NITROPHENYL)METHOXY]PHENYL]METHYL]-4-THIAZOLIDINECARBOXYLIC ACID ETHYL ESTER Solubility (25°C)：   体外:   DMSO 80mg/mL (198.77mM) Ethanol Insoluble Water Insoluble 体内(现配现用):   ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 2.4847mL 12.4236mL 24.8472mL 5mM 0.4969mL 2.4847mL 4.9694mL 10mM 0.2485mL 1.2424mL 2.4847mL   生物活性 产品描述 一种选择性的NCX抑制剂 靶点 IC502.9μM (NCX1),16μM (NCX2),8.6μM (NCX3)   体外研究 SN 6 is a selective Na+/Ca2+ exchanger inhibitor,which inhibits the initial rate of 45Ca2+ uptake into NCX1,NCX2,and NCX3 transfectants with IC50 values of 2.9±0.12,16±1.1,and 8.6±0.27μM.SN 6 (up to 30μM) also less potently inhibits muscarinic acetylcholine receptor,with a higher IC50 of 18μM.SN 6 (0.3-30μM) completely inhibits the initial rate of Na+i-dependent 45Ca2+ uptake into Na+loaded sarcolemmal vesicles in a dose dependent manner (IC50,5.3±0.37μM).SN 6 (0.3-10μM) dose-dependently protects against the hypoxia/reoxygenation-induced LDH release in parental LLC-PK1 cells and NCX1 transfectants but not in K229Q transfectants.SN 6 (1-30μM) suppresses the bidirectional outward and inward INCX in a concentration-dependent manner,with IC50 values of 2.3μM and 1.9μM,respectively.SN 6 also inhibits bidirectional current (INCX) in a [Na+]i concentration-dependent manner,with IC50 values of 3.4μM,2.3μM,and 1.1μM at 10mM,20mM,and 30mM [Na+]i,respectively.SN 6 inhibits hypoxia/reoxygenation-induced LDH release with an IC50 value of 0.63±0.15μM in NCX1 transfectants.   推荐实验方法(仅供参考) 细胞实验:   Na+i-dependent 45Ca2+ uptake into cells expressing the wild-type or mutated exchangers are assayed.In brief,confluent transfectants in 24-well dishes are loaded with Na+ by incubation at 37℃ for 40 min in 0.5mL of balanced salt solution (BSS) (10mM HEPES/Tris,pH 7.4,146mM NaCl,4mM KCl,2mM MgCl2,0.1mM CaCl2,10mM glucose,and 0.1% bovine serum albumin) containing 1mM ouabain and 10μM monensin.45Ca2+ uptake is then initiated by switching the medium to Na+-free BSS (replacing NaCl with equimolar choline chloride) or to normal BSS,both of which contain 0.1mM 45CaCl2 (370 kBq/mL) and 1mM ouabain.After a 30-s incubation,45Ca2+ uptake is terminated by washing cells four times with an ice-cold solution containing 10mM HEPES/Tris,pH 7.4,120mM choline chloride,and 10mM LaCl3.Cells are then solubilized with 0.1 N NaOH,and aliquots are taken for determination of radioactivity and protein.When present, SN 6 and KB-R7943 are included in the medium 15 min before the start of 45Ca2+ uptake.
保存条件：-20℃

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