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MIM1  ≥98%

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包装规格：1mg 5mg 10mg 25mg in glass bottle
溶解性：溶于DMSO（50 mg/mL 超声）
产品描述：基本信息 产品编号：M10813 产品名称：MIM1 CAS: 509102-00-5   储存条件 粉末 2-8℃ 四年 分子式： C17H21N3O3S 溶于液体 -80℃(避光) 六个月 分子量 347.43 -20℃(避光) 一个月 化学名：      Solubility (25°C)   体外 DMSO   Ethanol   Water   体内（现配现用）     ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 2.8783mL 14.3914mL 28.7828mL 5mM 0.5757mL 2.8783mL 5.7566mL 10mM 0.2878mL 1.4391mL 2.8783mL   生物活性 产品描述 一种骨髓细胞因子 1 (Mcl-1) 抑制剂。 靶点/IC50 Mcl-1 体外研究 MIM-1 selectively targets the BH3 binding groove of Mcl-1, with Bak-dependent apoptotic activity. To estimate the sensitivity of HA and T98G cells to the apoptosis inhibitor MIM-1, the colorimetric MTT assay is used to detect cell viability and to determine the IC50 value. The IC50 value of the HA cell line is almost 5-fold lower (16.10µM) compared with the IC50 of the T98G cell line (80.20µM) after MIM-1 inhibitor treatment.   推荐实验方法(仅供参考) 细胞实验:   The IC50 value, defined as the concentration that reduces the global growth of cells by 50%, is determined for the apoptosis inhibitors ABT-737 and MIM-1, individually, for the human astrocyte (HA) and human GB (T98G) cell lines. The apoptosis inhibitor concentrations and treatment time periods are selected experimentally according to preliminary experiments. The final ABT-737 treatment is performed with 10-fold increasing concentrations in the range of 0.001-100µM, and the final MIM1 treatment is performed with 4-fold increasing concentrations in the range of 0.4 400µM, for 48 h. The biochemical colorimetric MTT assay, based on the enzymatic conversion of MTT to a violet formazan salt, is used to assess the viability of the HA and T98G cells. Briefly, the cells in culture medium are seeded (3.5×103 cells/well for the HA cell line, 3.0×103 cells/well for T98G cell line) in 96-well microtiter plates. On the third day, the medium is changed to culture medium supplemented with the apoptosis inhibitor ABT-737 or MIM-1 at varied concentrations and incubation continued for another two days. After the treatment with the apoptosis inhibitors,cells are rinsed once with Dulbeccos phosphate buffer saline (DPBS) and further incubated in medium supplemented with 0.5mg/mL MTT in a humidified atmosphere for 6h.During a subsequent incubation for 16h in medium containing SDS [5% (w/v)], the precipitated formazan, the amount of which is proportional to the number of live cells, is solubilized. The absorbance of the formazan-containing solution is measured at 540nm using an ELISA plate reader. The absorbance is also determined for the medium of the control cells not exposed to the apoptosis inhibitors. The percentage of cell viability is calculated relative to the untreated control cells. The IC50 values are determined for both human brain cell lines after individual apoptosis inhibitor treatment.
保存条件：2-8℃

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