生物试剂
>
生化试剂
>
信号通路
> Saracatinib 促销
加入收藏 取消收藏

Saracatinib 促销  99.5778%

促销价¥{{model.attbuying.price}}

市场价¥0.00

累计销量0 累计评价0 人评论

别称 {{model.alias}}
中文名称 {{model.name}}
英文名称 {{model.enname}}
品牌 {{model.brand}}
CAS {{model.cas}}
分子式
分子量 {{model.molecularweight}}
MDL {{model.mdl}}
货号 {{model.procode}}
数量

+ -

件 库存{{model.attbuying.number}}件
立即购买 加入购物车 下载说明书

看了又看
包装规格：5mg 25mg 100mg in glass bottle
溶解性：溶于DMSO（28 mg/mL 超声）
产品描述：基本信息 产品编号：P10805 产品名称：PP1 CAS: 172889-26-8   储存条件 粉末 -20℃ 四年     分子式： C16H19N5 溶于液体 -80℃ 六个月 分子量 281.36 -20℃ 一个月 化学名：      Solubility (25°C)   体外 DMSO 5mg/mL (17.77mM) Ethanol Insoluble Water Insoluble 体内（现配现用）     ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 3.5542mL 17.7708mL 35.5417mL 5mM 0.7108mL 3.5542mL 7.1083mL 10mM 0.3554mL 1.7771mL 3.5542mL   生物活性 产品描述 一种有效的选择性 Src 家族抑制剂，抑制 Lck 和 Fyn，IC50 分别为 5 和 6nM。 靶点/IC50 IC50: 5nM (Lck), 6nM (Fyn), 250 nM (EGFR), >50μM (JAK2   体外研究 PP1 inhibits Lck (IC50=5nM) and FynT (IC50=6nM) in vitro at concentrations significantly lower than those required to inhibit ZAP-70 (IC50>100μM), JAK2 (IC50>50μM), the EGFR kinase, and protein kinase A. PP1 inhibits whole cell tyrosine phosphorylation and proliferation in T cells stimulated with anti-CD3 and mitogens. PP1 selectively inhibits IL-2 gene expression over GM-CSF and IL-2R gene induction in human T cells.   推荐实验方法(仅供参考) 激酶实验： Protein A-Sepharose beads (prepared as a 50% (w/v) suspension) are added to the antibody/lysate mixture at 250μL/mL and allowed to incubate for 30 min at 4°C. The beads are then washed twice in 1mL of lysis buffer and twice in 1 mL of kinase buffer (25 mM HEPES, 3mM MnCl2, 5mM MgCl2, and 100μM sodium orthovanadate) and resuspended to 50% (w/v) in kinase buffer. Twenty-five microliters of the bead suspension is added to each well of the enolase-coated 96-well high protein binding plate together with an appropriate concentration of compound and [γ-32P]ATP (25μL/well of a 200μCi/mL solution in kinase buffer). After incubation for 20 min at 20°C, 60μL of boiling 2× solubilization buffer containing 10mM ATP is added to the assay wells to terminate the reactions. Thirty microliters of the samples is removed from the wells, boiled for 5 min, and run on a 7.5% SDS-polyacrylamide gel. The gels are subsequently dried and exposed to Kodak X-AR film. For quantitation, films are scanned using a Molecular Dynamics laser scanner, and the optical density of the major substrate band, enolase p46, is determined. Concentrations of compound that causes 50% inhibition of enolase phosphorylation (IC50) are determined from a plot of the density versus concentration of compound. In companion experiments for measuring the activity of compounds against Lck, the assay plate is washed with two wash cycles on a Skatron harvester using 50mM EDTA, 1mM ATP. Scintillation fluid (100μL) is then added to the wells,and P incorporation is measured using a Pharmacia Biotech micro-β-counter. Concentrations of compound that causes 50% inhibition of enzyme activity (IC50) are determined from a plot of the percent inhibition of enzyme activity versus concentration of compound.   细胞实验:   Inhibition of anti-CD3-stimulated tyrosine phosphorylation in purified human peripheral blood T cells is measured as follows. All incubations are carried out at 37°C in an Eppendorf Thermomixer 5436 at a mixing setting of 11. Cells (1×106 in 100 μL of RPMI 1640 medium) are incubated for 15 min with drug prior to a 6-min incubation with 1 μg of anti-CD3/mL (antileu4, 100μg/mL). The final volume of the reaction is 115μL.Reactions are terminated by the addition of 57.5μL of 3×solubilization buffer incubated at 100°C prior to its addition. Samples are mixed, boiled for 5 min, and stored at -70°C. Western blots of these cell lysates, run on 10% SDS-polyacrylamide gels, are probed with a polyclonal anti-phosphotyrosine antibody, and immune complexes are detected with I-labeled protein A (ICN). For quantitation, films are scanned using a Molecular Dynamics laser scanner, and the optical densities of the major substrate band, p70,are quantitated in the presence of anti-CD3 (in the presence and absence of drug).Percent inhibition is calculated as follows: (1-(p70 optical density units in presence of drug/p70 units in absence of drug))×100.IC50 equals the concentration of compound at which 50% inhibition is measured.
保存条件：-20℃

猜你喜欢